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We aimed to investigate the effect of cell-cell dipole interactions in the equilibrium distributions in dielectrophoretic devices. We used a three dimensional coupled Monte Carlo-Poisson method to theoretically study the final distribution of a system of uncharged polarizable particles suspended in a static liquid medium under the action of an oscillating non-uniform electric field generated by polynomial electrodes. The simulated distributions have been compared with experimental ones observed in the case of MDA-MB-231 cells in the same operating conditions. The real and simulated distributions are consistent. In both cases the cells distribution near the electrodes is dominated by cell-cell dipole interactions which generate long chains. The agreement between real and simulated cells’ distributions demonstrate the method’s reliability. The distribution are dominated by cell-cell dipole interactions even at low density regimes (105 cell/ml). An improved estimate for the density threshold governing the interaction free regime is suggested.
BioMed Central
Publication date: 
1 Dec 2014

Massimo Camarda, Giuseppe Fisicaro, Ruggero Anzalone, Silvia Scalese, Alessandra Alberti, Francesco La Via, Antonino La Magna, Andrea Ballo, Gianluca Giustolisi, Luigi Minafra, Francesco P Cammarata, Valentina Bravatà, Giusi I Forte, Giorgio Russo, Maria C Gilardi

Biblio References: 
Volume: 13 Issue: 1 Pages: 1-10
Biomedical engineering online