The miniaturization of Real Time PCR (qPCR) systems is a crucial point towards the development of “genetic point-of-care” (PoC) that are able to offer sample-in-answer-out diagnostic analysis. Centralized laboratories and specialized staffs are needed for conventional DNA analysis. To solve this issue, we propose an innovative easy-to-use PoC technology based on a Lab-on-Disk miniaturized system, integrating nucleic acids extraction process based on Mags-Beads technology and detection based on qPCR. Lab-on-Disk system is composed by a polycarbonate disk with reagent-on-board for DNA extraction and a qPCR silicon-chip. A customized reader integrating electronic and optical modules was developed for driving the polycarbonate disk. Here we present results in the detection of Hepatitis B Virus (HBV) genome.
21 Feb 2018
Convegno Nazionale Sensori