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We examined the similarities and differences of the binding behavior of lysozyme (lys) with pectin varying in local charge density (blockwise distribution and statistical distribution of methoxyl groups, BP and SP) and similar degree of methoxylation (DM). The interaction at ionic strength I = 0.01 and pH 5.1 was found to be mainly electrostatic, associated with an exothermic enthalpy change. BP which is more inclined to self-association binds lys forming strongly associated complex particles with average sizes much larger (10–20 μm) than that of SP. The latter formed with lys small (0.6–3 μm) complex particles or liquid droplets depending on DM. The critical Iset and pHset values for BP/lys systems, above which complexes do not form, are not sensitive to DM (approximately 0.1 and 11.5 respectively), whereas for SP/lys systems the critical Iset and pHset values decrease significantly with increasing DM. The effect …
Publication date: 
1 Feb 2020

Yurij A Antonov, Irina L Zhuravleva, Miete Celus, Clare Kyomugasho, Salvatore Lombardo, Wim Thielemans, Marc Hendrickx, Paula Moldenaers, Ruth Cardinaels

Biblio References: 
Volume: 99 Pages: 105345
Food Hydrocolloids